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目的通过大鼠子宫增重试验和Hershberger试验研究农药联苯菊酯(BIF)的内分泌干扰作用。方法子宫增重试验中,60只雌性SD大鼠随机分为6组,每组10只:BIF低、中、高剂量组分别经口灌胃给予1.47、4.41和13.23mg/kgBW的BIF;溶剂对照组经口灌胃给予玉米油;17α-乙炔雌二醇(EE)经口阳性组经口灌胃给予1.0μg/kg BW的EE,EE经皮阳性组经口灌胃给予玉米油并皮下注射0.6μg/kg BW的EE。连续给药3天后,测定大鼠子宫湿重、干重,计算其子宫系数,并观察病理变化。在Hershberger试验中,60只去势雄性SD大鼠随机分为6组,每组10只:BIF低、中、高剂量组分别经口灌胃给予1.4、4.2和12.6mg/kg BW的BIF,阳性对照组经口灌胃给予3.0mg/kg BW的氟他胺,溶剂对照组和丙酸睾酮对照组(TP组)经口灌胃给予玉米油,除溶剂对照组外,其余各组均皮下注射给予0.2mg/kgBW丙酸睾酮。所有剂量组连续给药10天后,称量腹侧前列腺(VP)、精囊腺和凝固腺(SVCG)、肛提肌加球海绵体肌(LABC)、阴茎头(GP)、尿道球腺(COW)、肝、肾、肾上腺的重量,计算其脏器系数,测定血清中三碘甲腺原氨酸(T3)和甲状腺素(T4)水平。结果子宫增重试验中,与溶剂对照组相比,BIF高剂量组子宫湿重系数和干重系数显著增加(P<0.05)。组织病理学检查发现,BIF中、高剂量组子宫内膜增生、增厚,子宫内膜上皮细胞高度增高(P<0.05)。Hershberger试验,与TP组相比,BIF高剂量组VP、LABC重量显著减轻,T3、T4水平显著降低(P<0.05)。结论子宫增重试验和Hershberger试验提示BIF具有潜在的内分泌干扰作用。
Objective To study the endocrine disrupting effect of bifenthrin (BIF) by rat uterine weight gain test and Hershberger test. Methods: Sixty female SD rats were randomly divided into six groups (10 rats in each group). The BIF low, medium and high dose groups were orally administered with BIF of 1.47, 4.41 and 13.23 mg / The control group was given orally gavage with corn oil. EE of 17α-ethinyl estradiol (EE) group was orally administered orally with 1.0 μg / kg BW of EE. EE percutaneous positive group was orally administered with corn oil and subcutaneously EE at 0.6 [mu] g / kg BW was injected. Three days after continuous administration, the uterus wet weight and dry weight were determined, the uterine coefficient was calculated, and the pathological changes were observed. In the Hershberger test, 60 castrated male SD rats were randomly divided into 6 groups with 10 rats in each group: BIF in low, medium and high doses of BIF were orally administered with 1.4, 4.2 and 12.6 mg / kg BW of BIF respectively, The positive control group was orally administered with 3.0 mg / kg BW of flutamide, and the solvent control group and the testosterone propionate control group (TP group) were orally administered with corn oil. Except for the solvent control group, the other groups were subcutaneously Injection of 0.2 mg / kg BW of testosterone propionate. Ten days after continuous administration of all doses, ventral prostate (VP), seminal vesicle and coagulated gland (SVCG), levator ani muscle labialis (LABC), penis head (GP) ), The weight of liver, kidney and adrenal gland, the organ coefficient was calculated and the levels of triiodothyronine (T3) and thyroxine (T4) in serum were measured. Results In uterine weight gain test, the uterine wet weight coefficient and dry weight index of BIF high dose group were significantly higher than that of solvent control group (P <0.05). Histopathological examination revealed that endometrial hyperplasia and thickening of endometrial hyperplasia and endometrial epithelial cells in BIF medium and high dose groups were significantly increased (P <0.05). Hershberger test, compared with TP group, BIF high dose group VP, LABC weight was significantly reduced, T3, T4 levels were significantly lower (P <0.05). Conclusions Uterine weight gain test and Hershberger test suggest that BIF has potential endocrine disrupting effect.