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从用于接种猕猴的受ＨＣＶ感染的２份献血员的血清，第一代猕猴感染ＨＣＶ１１个月的１＃食蟹猴血清和第二代猕猴受第一代猕猴ＨＣＶ血清感染ＨＣＶ３个月后的１４＃恒河猴血清，提取ＫＮＡ，用自行设计的ＨＣＶ５＇非编码区和核心区（５＇ＮＴＲ—Ｃ区）引物进行逆转录ＰＣＲ，将扩增的７７９ｂｐｃＤＮＡ片段克隆到ｐＵＣ１９质粒上；每一株挑３个克隆用双脱氧链终止法测定其序列并矫正偏差。４株ＨＣＶ的５＇ＮＴＲ—Ｃ区序列，原代人Ａ株（ＣＸ１）ｃＤＮＡ全长７７９ｂｐ，原代人Ｂ株（ＣＸ２）ｃＤＮＡ全长７７８ｂｐ，第一代猕猴株（ＣＸ３）ｃＤＮＡ全长７７６ｂｐ，第二代猕猴株（ＣＸ４）ｃＤＮＡ全长均为７７７ｂｐ，ＣＸ１株和ＣＸ４株均在５＇ＮＴＲｎｔ—２１６有—Ｃ的插入，ＣＸ３和ＣＸ４Ｃ区ｎｔ３８５—３８７处的３个硷基缺失；ＣＸ１株与ＣＸ２、ＣＸ３、ＣＸ４比较，同源性分别为９８．０７％。９６．１５％、９５．２５％：ＣＸ２与ＣＸ３、ＣＸ４的同源性分别为９６．２８％、９５．７６％；ＣＸ３与ＣＸ４的同源性为９７．５６％。由克隆的ＨＣＶＣ区ｃＤＮＡ推导的氨基酸序列，从ＨＣＶ的启始密码起，ＣＸ１株、ＣＸ２株序列全长１６８个氨基酸，ＣＸ３株和ＣＸ４株序列全长
From the first two generations of cynomolgus monkeys infected with HCV # 1 cynomolgus serum infected with HCV and the second generation cynomolgus monkeys infected with HCV for 3 months with HCV 14 # rhesus monkeys serum was extracted KNA, with its own design of HCV 5 ’non-coding region and the core region (5’NTR-C region) primer reverse transcription PCR, the amplified 779bp cDNA fragment was cloned into pUC19 plasmid; each Three clones were picked and their sequences were determined by dideoxy chain termination and the deviations were corrected. The full-length cDNA of CX3 was 779bp. The full-length cDNA of CX2 was 778bp in length. The first generation cDNA of CX3 was 776bp , The second generation of CX4 cDNA was 777bp in length, both CX1 and CX4 were inserted in the 5’-NTRnt-216-C, and three of the CX3 and CX4C nt385-387 were deleted; CX1 Strains compared with CX2, CX3, CX4, the homology was 98.07%. 96.15% and 95.25% respectively. The homologies between CX2 and CX3 and CX4 were 96.28% and 95.76% respectively. The homology between CX3 and CX4 was 97.56%. The deduced amino acid sequence of the cloned HCVC region cDNA has a total length of 168 amino acids from CX1 strain and CX2 strain starting from the initiation codon of HCV,