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目的:探讨淫羊藿苷对抗体外高浓度葡萄糖诱导人脐静脉内皮细胞的损伤及其机制。方法人脐静脉内皮细胞株分别培养在含5.5mmol/L葡萄糖(正常对照组)、5.5mmol/L葡萄糖+24.5mmol/L甘露醇(高渗对照组)、30mmol/L葡萄糖(高糖损伤组)、30mmol/L葡萄糖+10μmol/L淫羊藿苷(低剂量淫羊藿苷组)和30mmol/L葡萄糖+100μmol/L淫羊藿苷(高剂量淫羊藿苷组)的培养基中培养48h,分别进行细胞活力、细胞培养上清液乳酸脱氢酶及一氧化氮含量、细胞丙二醛含量及谷胱甘肽-过氧化物酶活力的测定。结果:高糖损伤组及低、高剂量淫羊藿苷组的细胞活力、乳酸脱氢酶、一氧化氮、丙二醛含量及谷胱甘肽-过氧化物酶活力与正常对照组比差异均有统计学意义(P<0.01);低、高剂量淫羊藿苷组的细胞活力、乳酸脱氢酶、一氧化氮、丙二醛含量及谷胱甘肽-过氧化物酶活力与高糖损伤组比差异均有统计学意义(P<0.01)。高渗对照组各项指标与正常对照组相比无显著性差异。结论淫羊藿苷对体外高糖损伤的内皮细胞具有保护作用,其作用机制可能通过提高内皮细胞抗氧化酶活力,维持NO正常水平;减少细胞膜脂质过氧化损伤,维持膜完整性而实现的。
Objective: To investigate the effects of icariin on the injury of human umbilical vein endothelial cells induced by high concentration of glucose and its mechanism. Methods Human umbilical vein endothelial cell lines were cultured in the medium containing 5.5 mmol / L glucose (normal control group), 5.5 mmol / L glucose + 24.5 mmol / L mannitol (hypertonic control group), 30 mmol / L glucose ), 30mmol / L glucose + 10μmol / L icariin (low dose icariin group) and 30mmol / L glucose + 100μmol / l icariin (high dose icariin group) 48h. The cell viability, lactate dehydrogenase and nitric oxide contents in cell culture supernatants, MDA content in cells and glutathione peroxidase activity were measured respectively. Results: The cell viability, lactate dehydrogenase, nitric oxide, malondialdehyde and glutathione peroxidase activity in high glucose group and low and high dose icariin group were significantly lower than those in normal control group (P <0.01). The cell viability, lactate dehydrogenase, nitric oxide, malondialdehyde and glutathione peroxidase activities of low and high dose icariin group were significantly higher than those of high There was significant difference in glucose injury group (P <0.01). The indexes of hypertonic control group showed no significant difference compared with the normal control group. Conclusions Icariin has a protective effect on endothelial cells damaged by high glucose in vitro, and its mechanism may be through increasing the antioxidant enzyme activity of endothelial cells, maintaining the normal level of NO, reducing lipid peroxidation damage and maintaining membrane integrity .