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选用耐盐与丰产、不耐盐两套亲本品种,按不完全双列杂交设计,组配杂交组合,其F_1花药在筛选培养基(加0.3％NaCl)上诱导筛选耐盐突变体,摸清在诱导筛选过程中的主要遗传参数。结果表明:诱导筛选耐盐突变体的频率高低与亲本品种耐盐特性关系密切。就亲本品种耐盐性诱导率性状而言、亲本品种间的一般配合力高低差异明显。耐盐性诱导率的广义遗传力为97.9％,说明F_1的耐盐突变体诱导率的变异,是以遗传变异为主,受环境影响甚微;狭义遗传力为65.87％,表明加性遗传方差(遗传变异的可固定部分)占表现型方差比例中的比重较大。因此,依品种耐盐特性的表现选配杂交亲本,提高耐盐突变体诱导效率是有明显效果的。
Selection of salt-tolerant and high yielding, salt-tolerant two parental varieties, incomplete dialleaf hybrid design, combination of hybrid combinations, the F_1 anther screening of salt-tolerant mutants in the screening medium (plus 0.3% NaCl), find out The major genetic parameters in inducing the screening process. The results showed that the frequency of salt-tolerant mutagenesis was closely related to the salt tolerance of the parental varieties. In terms of the salt tolerance trait traits of parental varieties, there are significant differences in the general combining ability of the parental varieties. The general heritability of salt tolerance induction rate was 97.9%, indicating that the variation of induction rate of salt-tolerant mutants of F 1 was mainly genetic variation with little environmental impact. The narrow heritability was 65.87%, which indicated that additive genetic variance (Fixed part of genetic variation) accounted for a larger proportion of the proportion of variance in the phenotype. Therefore, according to the performance of varieties of salt tolerance matching parent selection to improve salt-induced mutagenesis efficiency has a significant effect.