论文部分内容阅读
目的了解妊娠中晚期和哺乳期接触磺胺二甲嘧啶对子代大鼠海马内神经生长因子(NGF)和脑源性神经营养因子(BDNF)的影响。方法清洁级SD母鼠在受孕第7日起至哺乳期结束分别每日喂饲磺胺二甲嘧啶50、100和200mg/kg,取其30日龄仔鼠的大脑进行免疫组化染色,观察海马内NGF和BDNF的表达。结果大脑的免疫组化分析发现,50、100、200 mg/kg组子代大鼠海马CA1和CA3区NGF表达的积分光密度值显著低于对照组(P<0.05),200 mg/kg剂量组BDNF表达的积分光密度值显著低于对照组(P<0.05)。结论磺胺二甲嘧啶喂饲染毒围生期母鼠,可能通过降低大鼠体内的甲状腺素浓度使脑组织NGF、BDNF表达下调,进而影响大脑的正常发育及功能。
Objective To investigate the effect of exposure to sulfamethazine on the expression of nerve growth factor (NGF) and brain-derived neurotrophic factor (BDNF) in the hippocampus of offspring in late pregnancy and lactation. Methods Clean SD rats were fed with 50, 100 and 200 mg / kg sulfamethazine respectively from the 7th day of pregnancy to the end of the lactation. The brain of 30-day-old offspring rats were harvested for immunohistochemical staining. The hippocampus Expression of NGF and BDNF. Results The immunohistochemical analysis of brain showed that the integral optical density of NGF expression in the hippocampal CA1 and CA3 of the hippocampus of 50,100,200 mg / kg group was significantly lower than that of the control group (P <0.05), 200 mg / kg dose The integral optical density of BDNF expression group was significantly lower than that of the control group (P <0.05). Conclusions Sulfamethazine can reduce the levels of thyroxine in rats and reduce the expression of NGF and BDNF in the brain, which may affect the normal development and function of the brain.