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目的:经眼神经注入DiI研究小鼠三叉神经节的形态学结构。方法:小鼠10只,体重25-30克,雌雄不拘,进行灌注固定后,在外科显微镜下开颅并确认三叉神经节和眼神经,分别于双侧眼神经植入DiI染色晶体。37℃恒温箱放置3个月,待DiI染色晶体扩散后,取出植入DiI染色晶体的眼神经和三叉神经节,再根据神经走向切片,通过荧光显微镜观察DiI染色晶体在三叉神经节内的分布。结果:眼神经离三叉神经节约1 cm处植入DiI染色晶体后,应用荧光显微镜明视野观察,均可见到高密度标记的眼神经纤维,行向后内,穿经眶上裂入颅。逐步靠近三叉神经节外上方,并进入三叉神经节内,眼神经标记的神经元位于三叉神经节的前内侧。在三叉神经节内可见到DiI标记的神经节细胞及神经纤维。神经纤维平行致密排列,并被神经节细胞神经纤维分隔成群或簇。神经节细胞呈圆形和卵圆形,大小不一,部分节细胞呈蜂窝状排列。亦可见神经元的突起,有的呈螺旋状连于胞体,有的呈线状连于胞体,并可见到双极神经元。结论:小鼠经眼神经注入DiI后,三叉神经节细胞和神经纤维的排列循序跟其他动物基本一致。
OBJECTIVE: To study the morphological structure of the trigeminal ganglion in mice via intraocular injection of DiI. METHODS: Ten mice, weighing 25-30 g, were randomly divided into male and female mice. Perfusion fixation was performed. The cranial craniotomy was performed under a surgical microscope and the trigeminal ganglion and the oculomotor nerve were identified. DiI-stained crystals were implanted into the bilateral ocular nerves respectively. 37 ℃ incubator placed 3 months until the DiI staining crystal diffusion, remove the implanted DiI staining of the ocular and trigeminal ganglion, and then slice according to the nerve to observe the distribution of DiI staining crystal in the trigeminal ganglion by fluorescence microscopy . Results: After the ocular nerve was implanted with DiI-stained crystal at a distance of 1 cm from the trigeminal nerve, high-density labeled oculomotor fibers could be seen under the bright field of fluorescence microscope. Gradually close to the top of the trigeminal ganglion, and into the trigeminal ganglion, neurons labeled ocular neurons in the medial trigeminal ganglion ago. DiI-labeled ganglion cells and nerve fibers can be seen in the trigeminal ganglion. Nerve fibers arranged in parallel and dense, and ganglion cells separated by nerve fibers into clusters or clusters. Ganglion cells were round and oval, different sizes, part of the cells were arranged in honeycomb. Neurons can also be seen protruding, and some were spirally connected to the cell body, and some were linear even in the cell body, and can be seen bipolar neurons. Conclusion: The order of arrangement of trigeminal ganglion cells and nerve fibers is basically the same with that of other animals.