论文部分内容阅读
目的:探讨细胞核增殖抗原ki-67在子宫内膜异位症中的表达和子宫内膜细胞增殖能力对子宫内膜异位症发病的影响。方法:应用免疫组化SP法检测58例子宫内膜异位症(EMS组)患者(包括36例卵巢子宫内膜异位症和22例子宫腺肌症)的异位内膜和在位内膜及15例子宫肌瘤患者子宫内膜(对照组)中ki-67的表达情况,以增殖指数为统计指标。结果:卵巢EMS和子宫腺肌症的在位和异位内膜ki-67的表达差异均无统计学意义;对照组子宫内膜和EMS组在位内膜的腺体和间质的ki-67表达在增生期均显著高于分泌期,EMS异位内膜增殖指数在增生期和分泌期差异无统计学意义(P>0.05);EMS异位内膜ki-67增殖指数在增殖期低于在位内膜,但在分泌期则显著高于在位内膜(P<0.01);EMS异位内膜腺体Ⅰ～Ⅱ期时的ki-67表达高于Ⅲ、Ⅳ期(P<0.05),但在位内膜的ki-67表达在不同临床分期中差异无统计学意义(P>0.05)。结论:ki-67增殖指数反映了子宫内膜细胞的增殖活性,EMS异位内膜细胞持续增殖活跃可能在子宫内膜异位症发生、发展中起重要作用。
Objective: To investigate the expression of ki-67 in endometriosis and the effect of proliferation of endometrial cells on the pathogenesis of endometriosis. Methods: Immunohistochemical SP method was used to detect the ectopic endometrium of 58 patients with endometriosis (EMS group), including 36 cases of ovarian endometriosis and 22 cases of adenomyosis Membrane and 15 cases of uterine fibroids endometrial (control group) ki-67 expression, the proliferation index as a statistical indicator. Results: The expression of ki-67 in eutopic and ectopic endometrium of ovarian EMS and adenomyosis had no statistical significance. In the endometrium and EMS group, the expression of ki- 67 expression in proliferative phase were significantly higher than the secretory phase, EMS ectopic endometrial proliferation index in proliferation and secretory phase difference was not statistically significant (P> 0.05); EMS ectopic endometrial ki-67 proliferation index in the proliferative phase low In eutopic endometrium, but significantly higher in eutopic endometrium than in eutopic endometrium (P <0.01). The expression of ki-67 in stage Ⅰ ~ Ⅱ ectopic endometrial glands was higher than that in stage Ⅲ and Ⅳ (P < 0.05). However, there was no significant difference in ki-67 expression in eutopic endometrium among different clinical stages (P> 0.05). Conclusion: Proliferation index of ki-67 reflects the proliferative activity of endometrial cells, and the ectopic endometrial cells of EMs proliferate continuously may play an important role in the occurrence and development of endometriosis.