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水孔蛋白(AQPs,aquaporins)是高效转运水分子的膜内在蛋白,具有丰富的多样性,在调控植物的水分关系中有重要作用。本研究利用西洋梨(Pyrus communis L.‘Bartlett’)基因组数据库,通过生物信息学手段鉴定西洋梨PcAQPs基因家族成员;并利用MEGA 6.0.5软件,采用邻接法构建系统发育树;利用GSDS 2.0软件进行基因结构分析,MEME程序进行Motif分析,AgBase v2.00程序进行GO分析;采用半定量RT-PCR技术研究PcAQPs基因组织表达情况。结果表明,西洋梨基因组中共有54个PcAQPs家族成员,均含有AQP特征结构域和保守的Motif基序,根据基因结构及系统进化分析可分为PIP、TIP、NIP和SIP等4个亚家族。不同基因间结构差别较大,但聚类关系较近的基因其结构类似。GO分析发现,多数PcAQPs基因具有转运蛋白活性,参与物质转运、应激反应、发育和代谢等生物学过程,但不同亚家族成员构成的细胞组分及参与的生物学过程具有明显差异。半定量结果表明,大多数PcAQPs基因在根、茎、叶和果实中均有表达,而且不同基因家族、不同基因间的组织表达模式存在差异。该研究为今后西洋梨PcAQPs基因的克隆和功能分析奠定了基础。
Aquaporins (AQPs), a membrane-bound protein that efficiently transports water molecules, are rich in diversity and play an important role in the regulation of plant water. In this study, we used the genomic database of Pyrus communis L.’Bartlett ’to identify the members of PcAQPs gene family by bioinformatics. The phylogenetic tree was constructed by using MEGA 6.0.5 software, The gene structure analysis, MEME program Motif analysis, AgBase v2.00 program GO analysis; using semi-quantitative RT-PCR technology to study PcAQPs gene expression. The results showed that there are 54 PcAQPs family members in the genome of Pear, which all contain the AQP signature domain and the conserved motif motif. According to the gene structure and phylogenetic analysis, the 4 subfamilies of PIP, TIP, NIP and SIP can be divided into four subfamilies. The structure of different genes vary greatly, but the clustering of genes closer to their similar structure. GO analysis found that most PcAQPs have transporter activity involved in biological processes such as substance transport, stress response, development and metabolism. However, the cellular components of different subfamilies and their biological processes are obviously different. Semi-quantitative results showed that most PcAQPs genes were expressed in roots, stems, leaves and fruits, and there were differences in the expression patterns of different gene families and different genes. This study laid the foundation for the cloning and functional analysis of PcAQPs in Pear.