Ca(NO3)2胁迫对茄子嫁接苗叶片活性氧代谢和膜脂过氧化的影响

来源 :江苏农业科学 | 被引量 : 0次 | 上传用户:fabiosyn
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以日本引进的茄子设施栽培专用耐盐品种Torvum Vigor为砧木,栽培品种苏崎茄为接穗,用营养液栽培,对80 mmol/L Ca(NO3)2胁迫下茄子嫁接苗和自根苗叶片抗氧化酶活性、活性氧代谢和膜脂过氧化的变化进行了比较。结果表明,Ca(NO3)2胁迫下茄子嫁接苗株高和生物量积累显著高于自根苗,叶片抗氧化酶活性显著高于自根苗,叶片O2.-产生速率与H2O2含量显著低于自根苗,叶片MDA含量与电解质渗透率显著低于自根苗。因此,Ca(NO3)2胁迫显著降低了幼苗叶片抗氧化酶活性,增加了O2.-产生速率、H2O2与MDA含量及电解质渗透率,但嫁接苗受Ca(NO3)2胁迫的抑制程度显著小于自根苗,良好的活性氧清除机制保护了植株细胞膜的完整性,使嫁接苗在Ca(NO3)2胁迫下表现出较高忍耐力。 In this paper, Torvum Vigor, a salt-tolerant cultivar for eggplant plant introduced from Japan, was used as the rootstock. The cultivated cultivar ’Kusuaki’ was used as a scion and was cultivated with nutrient solution. The antioxidant activity of grafted eggplant seedlings and self-rooted seedlings under 80 mmol / L Ca Changes in enzyme activity, active oxygen metabolism and membrane lipid peroxidation were compared. The results showed that the plant height and biomass accumulation of grafted eggplant under Ca (NO3) 2 stress was significantly higher than that of self-rooted shoots, and the antioxidant enzyme activity of leaves was significantly higher than that of self-rooted seedlings. The production rate and H2O2 content were significantly lower than those of self- , Leaf MDA content and electrolyte permeability were significantly lower than self-rooted seedlings. Therefore, Ca (NO3) 2 stress significantly reduced antioxidant enzyme activity, increased O2.- production rate, H2O2 and MDA content and electrolyte permeability of seedling leaves, but the inhibition of grafted seedlings under Ca (NO3) 2 stress was significantly less than Since roots and shoots, the mechanism of active oxygen scavenging protects the integrity of plant cell membrane, so that the grafted seedlings show higher endurance under Ca (NO3) 2 stress.
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