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目的:观察survivin特异性RNAi载体对胰腺癌PANC-1细胞中survivin mRNA和蛋白的表达的抑制作用。方法:将U6启动子驱动的survivin特异性RNAi质粒载体和阴性对照质粒分别转染PANC-1细胞,用RT-PCR和Western blot分别检测survivin mRNA和蛋白的表达。结果:转染survivin特异性RNAi载体24 h和48 h后,PANC-1细胞survivin mRNA表达抑制率分别为(52.67±3.51)%和(75.33±3.06)%,蛋白表达抑制率分别为(58.00±3.61)%和(76.67±4.73)%;与阴性对照组比较,差异均有统计学意义(均P<0.05)。结论:以U6为启动子的survivin的RNAi载体,能有效地抑制胰腺癌细胞株PANC-1中survivin的表达。
Objective: To observe the inhibitory effect of survivin-specific RNAi vector on the expression of survivin mRNA and protein in pancreatic cancer PANC-1 cells. Methods: The survivin-specific RNAi vector driven by U6 promoter and negative control plasmid were transfected into PANC-1 cells respectively. The expression of survivin mRNA and protein was detected by RT-PCR and Western blot. Results: The expression of survivin mRNA in PANC-1 cells was (52.67±3.51)% and (75.33±3.06)% respectively after transfection with survivin-specific RNAi vector for 24 h and 48 h. The inhibition rate of protein expression was (58.00±) 3.61)% and (76.67±4.73)%; Compared with the negative control group, the differences were statistically significant (all P<0.05). Conclusion: The survivin RNAi vector with U6 promoter can effectively inhibit the expression of survivin in pancreatic cancer cell line PANC-1.