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目的:观察中医不同治法对骨质疏松症大鼠骨骼、骨骼肌印第安刺猬蛋白(Ihh)含量变化的影响,为临床中医防治骨质疏松症的提供理论依据。方法:将SD雌性大鼠分为正常组、模型组、补肾组、健脾组、活血组。采用酶联免疫法测定小鼠骨骼、骨骼肌Ihh含量浓度变化。结果:(1)与正常组比较,模型组大鼠骨骼的Ihh含量降低明显(P<0.01);与模型组比较,补肾组和健脾组骨骼的Ihh含量明显升高(P<0.01)。(2)与正常组比较,模型组大鼠骨骼肌的Ihh含量显著降低(P<0.01);与模型组比较,补肾组和健脾组骨骼肌的Ihh含量明显升高(P<0.01)。结论:(1)骨质疏松症的形成,可能与骨骼、骨骼肌协调性下降有关。(2)补肾、健脾方法通过提高骨质疏松症大鼠的骨骼、骨骼肌Ihh含量,对骨质疏松症具有一定的防治作用。
Objective: To observe the effect of different treatment of traditional Chinese medicine (TCM) on the content of Indian Hedgehog (Ihh) in skeletal and skeletal muscle of osteoporosis rats and to provide theoretical basis for clinical prevention and treatment of osteoporosis. Methods: SD female rats were divided into normal group, model group, Bushen group, Jianpi group and Huoxue group. The concentration of Ihh in bone and skeletal muscle of mice was measured by enzyme-linked immunosorbent assay. Results: (1) Compared with the normal group, the content of Ihh in the model group decreased significantly (P <0.01). Compared with the model group, the content of Ihh in the model group was significantly increased (P <0.01). (2) Compared with the normal group, the content of Ihh in the skeletal muscle of model group was significantly decreased (P <0.01). Compared with the model group, the Ihh content of skeletal muscle of Bushen group and Jianpi group was significantly increased (P <0.01). Conclusion: (1) The formation of osteoporosis may be related to the decreased coordination of skeletal and skeletal muscles. (2) The method of invigorating the kidney and invigorating the spleen can prevent and cure osteoporosis by increasing the content of Ihh in skeletal and skeletal muscle of osteoporosis rats.