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目的研究自含式压力蒸汽灭菌生物指示剂D值测定的影响因素,提高D值测定的准确性。方法使用生物指示剂抗力测定仪,对不同生产企业的自含式压力蒸汽灭菌生物指示剂进行D值测定,比较透气孔、培养基、放置方式、载样器材对D值测定的影响。结果透气孔、培养基、放置方式、载样器材对D值的最大影响率分别为6.9%、17.3%、29.9%、38.2%。结论放置方式和载样器材是影响自含式压力蒸汽灭菌生物指示剂D值测定的主要因素,建议用带孔支架以水平方式进行测定。
Objective To study the influential factors of D value determination of self-contained pressure steam sterilization biological indicator and to improve the accuracy of D value determination. Methods The bioassay instrument was used to measure the D value of self-contained pressure steam sterilization biological indicator of different manufacturing enterprises. The effects of air hole, medium, placement method and sample loading equipment on the determination of D value were compared. Results The maximum influence rate of air hole, medium, placement method and sample loading equipment on D value were 6.9%, 17.3%, 29.9% and 38.2% respectively. Conclusion The placement method and sample loading equipment are the main factors influencing the D value determination of self-contained pressure steam sterilization bioindicators. It is suggested that the perforated scaffold should be used for horizontal measurement.