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目的以小鼠肝脏细胞中的超氧化物歧化酶(SOD)、过氧化物酶(POD)、过氧化氢酶(CAT)的活力和丙二醛(MDA)含量为指标,探讨全氟辛烷磺酸钾(PFOS-K)对小鼠肝脏的氧化性损伤作用。方法用剂量为3、6、12和24mg/kg(按体重计)4个浓度的PFOS-K混悬液,分别给小鼠灌胃染毒,20 d后检测肝脏脏器系数,以及肝脏中SOD、POD、CAT的活力和MDA含量变化。结果在染毒剂量3～24 mg/kg浓度范围内,小鼠体重呈现逐渐降低趋势(r=-0.96),肝脏重量(r=0.92)和肝脏脏器系数(r=0.968)逐渐表现出升高趋势;3种抗氧化酶活力则随着剂量的升高表现出下降趋势(rSOD=-0.93,rPOD=-0.91,rCAT=-0.92),而丙二醛含量呈现上升趋势(r MDA=0.96)。结论在本试验条件下,PFOS-K对小鼠肝脏细胞具有氧化损伤作用。
Objective To investigate the activity of superoxide dismutase (SOD), peroxidase (POD), catalase (CAT) and malondialdehyde (MDA) Oxidative injury of mouse liver by potassium sulfonate (PFOS-K). Methods Four concentrations of PFOS-K suspension at 3, 6, 12 and 24 mg / kg (by weight) were intragastrically administered to mice, respectively, and liver organ coefficients were detected 20 days later, SOD, POD, CAT activity and MDA content changes. Results The body weight decreased gradually (r = -0.96), liver weight (r = 0.92) and liver organ coefficient (r = 0.968) gradually increased from 3 mg / kg to 24 mg / kg (RSOD = -0.93, rPOD = -0.91, rCAT = -0.92), while the content of malondialdehyde showed an upward trend (r MDA = 0.96 ). Conclusion Under the test conditions, PFOS-K has oxidative damage on mouse liver cells.