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目的已有实验证实三羟异黄酮(GST)对绝经后妇女具有较好的心血管保护作用。观察GST对去卵巢大鼠外周血白细胞介素6(IL-6)、肿瘤坏死因子α(TNF-α)和核转录因子κB(NF-κB)表达以及主动脉张力变化的影响,旨在探讨GST对绝经后女性心血管保护作用的机制。方法将40只成年雌性大鼠随机分成4组:假手术组(n=10),去卵巢组(n=10),雌激素组[20μg/(kg.d),n=10],GST组[200μg/(kg.d),n=10]。第8周末取标本,酶联免疫法测定大鼠血清IL-6、TNF-α和NF-κB水平。结果去卵巢组炎症标志物水平显著高于对照组[IL-6(95.8±16.2)比对照组(40.2±2.8)ng/L;TNF-α(60.3±6.4)比对照组(18.3±3.3)ng/L;NF-κB(11.9±3.3)比对照组(2.6±1.2)ng/L,均P<0.01]。补充GST后IL-6(43.4±3.7)ng/L、TNF-α(19.9±3.2)ng/L和NF-κB(2.2±1.0)ng/L水平均显著降低(均P<0.01)。去卵巢大鼠主动脉张力[最大收缩力(4.58±1.36)g]明显高于对照组[最大收缩力(1.83±0.95)g,P<0.01)],补充GST可明显降低动脉血管张力[最大收缩力(1.99±0.39)g,P<0.01]。结论GST能显著降低去卵巢大鼠外周血中IL-6、TNF-α和NF-κB水平和主动脉张力。
Objectives Experiments have confirmed that genistein (GST) has a good cardiovascular protective effect on postmenopausal women. To observe the effects of GST on the expression of interleukin 6 (IL-6), tumor necrosis factor α (TNF-α) and nuclear factor kappa B (NF-κB) in peripheral blood and aortic tension in ovariectomized rats Mechanism of cardiovascular protective effect of GST on postmenopausal women. Methods 40 adult female rats were randomly divided into 4 groups: sham operation group (n = 10), ovariectomized group (n = 10), estrogen group [20μg / (kg · d), n = 10] [200 μg / (kg.d), n = 10]. At the end of the 8th week, serum IL-6, TNF-α and NF-κB levels were measured by enzyme-linked immunosorbent assay. Results The levels of inflammatory markers in ovariectomized group were significantly higher than those in control group (95.8 ± 16.2 vs. 40.2 ± 2.8 ng / L, 60.3 ± 6.4 vs. 18.3 ± 3.3, ng / L; NF-κB (11.9 ± 3.3) vs control group (2.6 ± 1.2) ng / L, all P <0.01]. The levels of IL-6 (43.4 ± 3.7) ng / L, TNF-α (19.9 ± 3.2) ng / L and NF-κB (2.2 ± 1.0) ng / L were significantly decreased after GST supplementation (all P <0.01). Aortic tension (maximal contractility (4.58 ± 1.36) g] in ovariectomized rats was significantly higher than that in the control group [maximal contractility (1.83 ± 0.95) g, P <0.01). Supplementation of GST significantly reduced arterial vascular tone [maximal Contractility (1.99 ± 0.39) g, P <0.01]. Conclusion GST can significantly reduce the level of IL-6, TNF-α, NF-κB and aortic tension in ovariectomized rats.