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目的:探讨采用TLC鉴别方法区别部分中药生品与炮制品的可行性。方法:以TLC鉴别方法,采用适当方法制备供试品溶液,以5-羟甲基糠醛(5-HMF)为对照品,硅胶GF254薄层板或硅胶G薄层板层析,以石油醚(60~90℃)-乙酸乙酯(1∶1)为展开剂,紫外光灯(254 nm)下检视或喷以15%α-萘酚乙醇溶液,在90℃加热至斑点显色清晰,日光下检视。结果:本方法可区别地黄、黄精、山茱萸、女贞子、肉苁蓉和大黄的生品与炮制品,不能用于区别黄芩和五味子生品与炮制品。结论:在深入研究的基础上,可望用于部分中药生品与炮制品的鉴别。
Objective: To explore the feasibility of using TLC discriminating method to discriminate between some Chinese herbal medicines and processed products. Methods: Prepare the test solution by TLC identification method with appropriate method. Use 5-Hydroxymethylfurfural (5-HMF) as the reference substance, silica gel GF254 thin-layer plate or silica gel G thin-layer plate chromatography to petroleum ether ( 60~90°C)-ethyl acetate (1 : 1) as developing solvent, under UV light (254 nm), examined or sprayed with 15% α-naphthol ethanol solution, heated at 90°C until the spot color is clear, daylight Check it out. Results: This method can distinguish between raw yellow and yellow, spermatophyte, hawthorn, Ligustrum lucidum, Cistanche and rhubarb raw products and processed products, can not be used to distinguish between Huangqi and Schisandra raw products and processed products. Conclusion: Based on the in-depth study, it can be used for the identification of some Chinese herbal medicines and processed products.