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为阐明专利菌种Candida tropicalis Y1利用玉米秸秆产乙醇的能力,以未脱毒汽爆玉米秸秆为原料进行分步糖化发酵(SHF)及同步糖化发酵(SSF)产乙醇。研究结果表明,底物浓度为25%(w/v)、纤维素酶15FPU/(g底物)、纤维二糖酶Novozyme 188 20IU/(g底物)、接种量5%(v/v)及不需添加任何外源营养物质,该菌在SHF过程中的乙醇浓度为38.75g/L乙醇,为理论产率的77.9%,在SSF过程中的乙醇浓度为40.53g/L;同时,SSF与SHF相比,SSF可得到更多的木糖醇,且毒性物质乙酸的浓度维持在较低水平。说明该专利菌株不但有较强的耐发酵抑制剂的能力,而且有较好的代谢葡萄糖和木糖分别产乙醇和木糖醇的能力。
In order to elucidate the ability of Candida tropicalis Y1 to produce ethanol by using corn stalk, stepwise saccharification and fermentation (SHF) and simultaneous saccharification and fermentation (SSF) of ethanol were carried out using unstripped steam explosion corn stalk as raw material. The results showed that the substrate concentration was 25% (w / v), cellulase 15 FPU / g substrate, cellobiase Novozyme 188 20 IU / g substrate, inoculum 5% (v / v) And without adding any exogenous nutrients, the concentration of ethanol in the SHF process was 38.75g / L ethanol, which was 77.9% of the theoretical yield and the ethanol concentration in the SSF process was 40.53g / L. Meanwhile, SSF Compared with SHF, SSF can get more xylitol, and the concentration of toxic substance acetic acid is maintained at a low level. This patented strain not only has a strong resistance to fermentation inhibitors, but also has a good ability to metabolize glucose and xylose to produce ethanol and xylitol respectively.