To compare the therapeutic effects of the mind-tranquilizing and menstruation-regulating acupuncture method with the routine acupuncture method in treating delayed menstrual cycle. Methods: 40 patients with delayed menstrual cycle were randomly divided into a treatment group of 23 cases (treated by the mind-tranquilizing and menstruation-regulating acupuncture method),and a control group of 17 cases (treated by the routine acupuncture method for delayed menstrual cycle due to stagnation of the liver-qi). The treatment involved three menstrual cycles. The evaluations were done by scoring the symptoms before treatment and at the end of each menstrual cycle. Results: After treatment,significant differences were found between the two groups in the therapeutic effects (P<0.05). Conclusion: The therapeutic effect of the mind-tranquilizing and menstruation-regulating acupuncture method is significantly superior to that of the routine acupuncture method for delayed menstrual cycle.