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昆明种小鼠随机分成７组，１～５组小鼠皮下注射二甲基肼（１，２－Ｄｉｍｅｔｈｙｌｈｙｄｒａｚｉｎｅ，ＤＭＨ），剂量２０ｍｇ／ｋｇｂｗ，每周一次，连续２０周。第２～５组于注射前一周开始灌胃儿茶素，每鼠每日分别为１ｍｇ、２ｍｇ、４ｍｇ、没食子酰表没食子儿茶素［（－）－Ｅｐｉｇａｌｌｏ－ｃａｔｅｃｈｉｎｇａｌｌａｔｅ］２ｍｇ，第６组给儿茶素３ｍｇ作为对照组，每周连续５次至２３周。第７组为溶剂对照组。２７周处死小鼠，结果显示：两组对照组小鼠未发现肿瘤。ＤＭＨ组大肠癌发生率为８０％，与２～５组比较明显升高（Ｐ＜０．００１），结果提示：（１）不同剂量的儿茶素都具有防癌作用，其机理可能与诱导ＳＯＤ等抗氧化剂的活性消除有害自由基有关；（２）其抑癌有效成分可能是多种儿茶素协同作用的结果；（３）儿茶素对个鼠无毒性作用。
Kunming mice were randomly divided into 7 groups. 1 to 5 mice were injected subcutaneously with 1,2-Dimethylhydrazine (DMH) at a dose of 20 mg / kg bw once a week for 20 weeks. Groups 2 to 5 were intragastrically administered with catechin one week prior to injection, with 1 mg, 2 mg, and 4 mg of epigallocatechin gallate [(-) - Epigallo-catechingallate], 2 mg Catechin 3mg as a control group, 5 times a week to 23 weeks. Group 7 is a solvent control group. Mice were sacrificed at 27 weeks and the results showed that no tumors were found in the two control groups. The incidence of colorectal cancer in DMH group was 80%, which was significantly higher than that in 2 ~ 5 group (P <0.001). The results suggest that: (1) Catechins with different doses all have anti-cancer effects, which may be related to induction SOD and other antioxidants to eliminate harmful free radicals; (2) its anti-cancer active ingredients may be the result of a variety of catechins synergistic effect; (3) catechins non-toxic effect on the mice.