本文报道用反相高效液相层析法测定人血浆中的阿米替林和去甲替林浓度。采用Ultrasphere CN柱,甲醇:0.01M磷酸盐缓冲液(75:25)为流动相,甲氯苯咪作内标,在254nm波长下监测洗提液,对萃取、分离等条件及影响因素进行了探讨。本法具快速、简便、灵敏、准确的特点,适于临床实验室作阿米替林和去甲替林的血药浓度监测及药物动力学研究之用。 This paper reports the determination of concentrations of amitriptyline and nortriptyline in human plasma by reversed-phase high performance liquid chromatography. Ultrasphere CN column, methanol: 0.01 M phosphate buffer (75:25) as the mobile phase, meclofenamate as an internal standard, at 254nm wavelength monitoring eluent, extraction, separation conditions and influencing factors Discussion. The method is rapid, simple, sensitive and accurate. It is suitable for monitoring blood concentration and pharmacokinetics of amitriptyline and nortriptyline in clinical laboratories.