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以正常人血淋巴细胞染色体ＤＮＡ为模板，ＰＣＲ扩增出神经营养因子４（ＮＴ４）编码基因．将所得基因片段重组于噬菌体载体Ｍ１３ｍｐ１８ＲＦ，筛选得到含人ＮＴ４基因的克隆．采用Ｓａｎｇｅｒ单链末端终止法测出其全部的核苷酸序列，该序列与国外文献所报道的完全一致．
Using normal human lymphocyte chromosome DNA as a template, a gene encoding neurotrophic factor 4 (NT4) was amplified by PCR. The resulting gene fragment was recombined on the phage vector M13mp18RF, and the clone containing the human NT4 gene was screened. Sanger’s single-stranded terminator method was used to detect the complete nucleotide sequence of the nucleotide sequence, which was completely consistent with that reported in foreign literature.