Objective To investigate the significance of nucleic tracing method on channel and point study and the action of points on meridian. Method Divide 39 patients with chronic cholecystitis into observing and control group. Observe nucleic trace dynamically first. After 28 hours, observe the effect of acupunctured channel and points and their adjacent tissue on the trace of nucleic. Result Therere significant differences in anastomosis rate and the change of variance of channel and points before and after acupuncture, which didnt happen in control group.Compared with pre acupuncture, the latent period of nucleic trace shortened obviously, migrating speed increased, local clearance improved,passing time shortened after acupuncture in observing group. Theres significant difference between pre and post acupuncture. In control group,except latent period theres no difference between changes of other 3 parameters. Conclusion Points have biological characteristics different from other common body tissues.