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按烛缸法对柏氏疟原虫(Plasmodium berghei)红内期进行了体外培养的初步研究。先后5次,每次连续培养7天。除培养的第2天,其原虫率稍有增长外,随后便逐日下降;与此同时,培养的第4天,环状体有增多外,其后也逐日减少,而未成熟裂殖体及成熟裂殖体相对地逐日增加。培养结果提示有1～2个周期的裂体增殖。另将第7天的培养物无菌注入健康正常小白鼠腹腔内,7天后血检,原虫率明显上升(>5％),各红内期形态均可见到,还偶见到配子体,鼠间继续人工传代,原虫活力不减,实验鼠均于1周左右自然致死,反映原虫经体外培养7天后对宿主仍具有很强的毒力。
A Preliminary Study on In Vitro Cultivation of Plasmodium berghei by the Candlestick Method in. Has 5 times, each time for 7 days. In addition to the second day of culture, the parasite rate increased slightly, and then decreased day by day. At the same time, on the 4th day of culture, the number of ring bodies increased and then decreased day by day. However, the immature schizonts and Mature schizonts relatively daily increase. Culture results suggest that there is 1 to 2 cycles of Riparian proliferation. On the other hand, the culture of the 7th day was aseptically injected into the peritoneal cavity of healthy and normal mice. After 7 days of blood test, the protozoal rate increased significantly (> 5%), and all red-phase morphologies were visible. Continue to artificial passage, Protozoa activity diminished, mice were naturally lethal about 1 week, reflecting the protozoa cultured in vitro 7 days after the host still has a strong virulence.