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目的:探讨青春期大鼠癫痫发作后海马齿状回颗粒细胞层神经细胞数量的变化。方法:选择健康4周龄雄性SD大鼠,应用氯化锂-匹罗卡品药物点燃造模,造模成功后根据取脑组织时间分为24 h组、2周组、4周组,并设相应的对照组。溴脱氧尿嘧啶核苷(BrdU)标记后免疫荧光染色,用激光共聚焦观察大鼠海马齿状回(DG)颗粒细胞层BrdU阳性细胞。结果:24 h和2周实验组BrdU阳性细胞显著增多,分别较对照组增加55.1%和39.6%,2周实验组比24 h实验组降低15.5%(P<0.05);4周实验组BrdU阳性细胞数较对照组无明显差异(P>0.05)。结论:青春期大鼠癫痫发作可引起海马齿状回颗粒层神经细胞增殖的升高,但随着时间的延长有下降的趋势,至4周左右神经细胞的增殖趋于正常。
Objective: To investigate the changes of the number of nerve cells in the granular layer of dentate gyrus in adolescent rats after epileptic seizures. Methods: Healthy 4-week-old male Sprague-Dawley rats were selected and injected with lithium chloride-pilocarpine to make models. After successful modeling, the rats were divided into 24 h group, 2 weeks group and 4 weeks group according to brain tissue time Set up the corresponding control group. BrdU labeling was used for immunofluorescence staining, and BrdU positive cells were detected by confocal laser scanning microscopy. Results: BrdU positive cells in experimental group increased significantly by 55.1% and 39.6% at 24 and 2 weeks, respectively, 15.5% (P <0.05) in experimental group at 24 and 2 weeks; BrdU positive at 4 weeks There was no significant difference in cell number between the two groups (P> 0.05). CONCLUSION: Epileptic seizures in adolescent rats can induce the proliferation of neuronal cells in the granular layer of dentate gyrus in the hippocampus. However, the neuronal cell proliferation tends to be normal after 4 weeks.