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目的观察秦艽不同配伍药对风湿热痹类风湿关节炎模型大鼠足跖肿胀度、关节炎症及血清类风湿因子、C-反应蛋白含量的影响。方法将80只SD大鼠随机分为空白组、Ⅱ型胶原组、病证模型组、阳性药组、单味秦艽组、秦艽威灵仙组、秦艽桑寄生组和秦艽防己组共8组,每组10只。采用Ⅱ型胶原诱导加风湿热环境因素刺激制备风湿热痹类风湿关节炎大鼠模型,连续18d。造模结束后各给药组给予制备的对应药液灌胃,空白组、Ⅱ型胶原组、病证模型组给予等量0.9%氯化钠溶液,连续21d。实验中每3d测一次足跖厚度,并计算足跖肿胀度;实验第38天进行关节炎指数评分,酶联免疫法测定各组大鼠血清类风湿因子、C-反应蛋白含量。结果与空白组比较,Ⅱ型胶原组和病证模型组的足跖肿胀度和关节炎指数明显升高(P<0.01);与病证模型组比较,各给药组足跖肿胀度和关节炎指数均有不同程度的降低,其中秦防组降低最为显著(P<0.01)。血清学检测结果显示,与空白组比较,Ⅱ型胶原组及病证模型组大鼠血清类风湿因子、C-反应蛋白含量明显增加;与病证模型组比较,各给药组大鼠血清类风湿因子、C-反应蛋白均有不同程度降低,其中,秦防组类风湿因子、C-反应蛋白含量下降尤为明显(P<0.01)。结论风湿热痹型类风湿关节炎,平寒相配效果优于平温、平平相配,实验结果基本符合中医临床“疗热以寒药”的治疗原则。
Objective To observe the effect of different compatibility drugs of Gentiana macrophylla on foot swelling, arthritis, serum rheumatoid factor and C-reactive protein in rheumatoid arthritis model rats with rheumatoid arthritis. Methods Eighty Sprague Dawley rats were randomly divided into 8 groups: blank group, type Ⅱ collagen group, disease model group, positive drug group, single genotype Qinhuang group, clematis genus Qinhuang group, Each group of 10. Type Ⅱ collagen-induced rheumatoid arthritis induced by environmental factors to stimulate rheumatoid arthritis rat model of rheumatoid arthritis, continuous 18d. After the model was finished, the corresponding drug solution was given to the rats in each administration group for gavage, blank group, type Ⅱ collagen group and model group with 0.9% sodium chloride solution for 21 days continuously. The thickness of the plantar paw was measured every three days in the experiment and the degree of paw edema was calculated. The arthritis index was scored on the 38th day and the content of serum rheumatoid factor and C-reactive protein in the rats in each group was measured by enzyme-linked immunosorbent assay. Results Compared with the blank group, the degree of paw edema and arthritis index in type Ⅱ collagen group and model group were significantly increased (P <0.01). Compared with the model group, the degree of paw edema and joint Inflammatory index decreased to varying degrees, of which Qin prevention group decreased the most significant (P <0.01). Serological tests showed that compared with the blank group, the serum levels of rheumatoid factor and C-reactive protein in type Ⅱ collagen group and model group were significantly increased; compared with the model group, the serum levels of each group Rheumatoid factor and C-reactive protein decreased to some extent. Among them, the contents of rheumatoid factor and C-reactive protein in Qin prevention group decreased significantly (P <0.01). Conclusion Rheumatoid Arthritis with paralysis of rheumatoid arthritis and Pinghan are better than those with flat temperature and flatness. The experimental results are in line with the principles of traditional Chinese medicine “treatment of fever with cold medicine”.