论文部分内容阅读
目的探讨结直肠癌远处转移患者免疫基因表达的变化趋势。方法从16例结直肠癌患者的原发癌肿瘤组织中提取mRNA,采用基因芯片技术检测8例有肝脏转移和无肝脏转移的结直肠癌患者的免疫基因表达。结果与无肝脏转移的结直肠癌患者相比,有肝脏转移的结直肠癌患者的肿瘤组织中有11条免疫基因即羧基肽酶D、高亲和力IgE Fc受体γ链、低亲和力IgG FcⅢa受体、游离脂肪酸受体2、白细胞介素-2γ链、受体型蛋白酪氨酸磷酸酶C、补体B因子、人类白细胞抗原复合物(HLA)-DMA、HLA-DMB、HLA-DQA1和颗粒酶B均表达下调。涉及功能变化包括免疫细胞的生长激活、信号传递、肿瘤免疫原性、细胞因子、受体、补体、肿瘤细胞凋亡等方面。结论在结直肠癌肝转移患者中免疫基因的表达普遍下调,从多种途径影响机体免疫功能的发挥,使癌细胞逃避机体免疫系统杀灭而在远处目标脏器生长、繁殖。
Objective To investigate the changes of immune gene expression in patients with distant metastasis of colorectal cancer. Methods mRNA was extracted from 16 cases of colorectal cancer patients with primary cancer. The gene expression of immune genes in 8 cases of colorectal cancer with and without liver metastasis was detected by gene chip technique. Results Compared with colorectal cancer patients without liver metastasis, there were 11 immune genes in the tumor tissues of patients with liver metastases, ie, carboxypeptidase D, high-affinity IgE Fc receptor γ chain and low affinity IgG FcⅢa receptor Body, free fatty acid receptor 2, interleukin -2 gamma chain, receptor tyrosine phosphatase C, complement B factor, human leukocyte antigen complex (HLA) -DMA, HLA-DMB, HLA-DQA1 and particles Enzyme B were down-regulated. Functional changes involved include growth and activation of immune cells, signaling, tumor immunogenicity, cytokines, receptors, complement, tumor cell apoptosis and so on. Conclusion The expression of immune genes in patients with liver metastasis of colorectal cancer is generally down-regulated, and the immune function is affected in many ways. Cancer cells can escape from the immune system and grow in the distant target organs.