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目的了解加替沙星正常剂量用药和非正常剂量用药方式对小鼠肠道菌群的影响。方法24只昆明小鼠随机分为3组,每组8只,对照组只灌胃生理盐水,另外2组分别按正常剂量和正常剂量的一半给小鼠灌胃加替沙星溶液,7d后无菌采取小鼠粪便,观察各组小鼠粪便中双歧杆菌、类杆菌、乳酸杆菌、大肠埃希菌及肠球菌数量。结果加替沙星灌胃7d后,与给药前相比,肠杆菌和肠球菌药敏结果无明显变化。灌胃前后肠杆菌对CIP、LEV、GAT、AM、CRO、AMK、NOR均敏感,肠球菌对AM、LEV、VA、GAT均敏感,对NOR、CIP、CRO敏感性不同。2种用药方式可导致大肠埃希菌数量显著减少(P<0.05),肠球菌数量稍有增加(P>0.05)。而双歧杆菌、乳酸杆菌和类杆菌数量无明显变化(P>0.05)。结论加替沙星2种用药方式短时期对肠道菌群影响不大(肠杆菌除外),不易产生耐药性。其杀灭肠杆菌的作用远大于对厌氧菌的作用。
Objective To investigate the effect of gatifloxacin on the intestinal microflora in normal and non-normal dosage groups. Methods Twenty-four Kunming mice were randomly divided into three groups, with 8 rats in each group. The control group was fed with normal saline only. The other two groups were given gatifloxacin solution by normal dose and normal dose, Aseptic mice feces were used to observe the fecal bifidobacteria, Bacteroides, Lactobacilli, Escherichia coli and enterococci in each group of mice. Results Gatifloxacin gavage 7d, compared with before administration, enterococci and enterococci drug sensitivity results no significant change. Enterobacter was sensitive to CIP, LEV, GAT, AM, CRO, AMK and NOR before and after gavage. Enterococcus was sensitive to AM, LEV, VA and GAT, but different to NOR, CIP and CRO. The two methods of administration led to a significant decrease in the number of Escherichia coli (P <0.05) and a slight increase in the number of Enterococci (P> 0.05). The number of Bifidobacterium, Lactobacillus and Bacteroides did not change significantly (P> 0.05). Conclusion Gatifloxacin has no effect on intestinal flora in a short period of time (except Enterobacteriaceae) in the two modes of administration, which is not easy to produce drug resistance. Its role in killing Enterobacteria far greater than the role of anaerobic bacteria.