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在温室内将提纯的ＴＭＶ和ＰＶＸ病毒，通过摩擦接种在普通烟（Ｎｉｃｏｔｉａｎａ ｔａｂａｃｕｍＬ．）上做为繁毒植物。通过ＰＥＧ沉淀和差速离心，获得提纯病毒。病毒含量分别为２１．４２ｍｇ／１００ｇ鲜重和２４．５０ｍｇ／１００ｇ鲜重。在紫外分光灯下测定ＯＤ值，分别在２４８ｎｍ（ＴＭＶ）和２４６ｎｍ（ＰＶＸ）为最低，２６０ｎｍ为最高。消光系数ＯＤ２６０／２８０分别为１．２０和１．２２。提纯抗原进行回接试验，指示植物产生明显症状，用Ｆｒｅｕｎｄ佐剂分三次肌肉免疫家兔，所得抗血清效价为１：２０４８，然后利用（ＮＨ_４）_２ＳＯ_４提取球蛋白，并利用ＨＲＰ进行酶标记，制备出的抗血清现已应用于生产。
The purified TMV and PVX viruses were inoculated on the normal tobacco (Nicotiana tabacum L.) as frictional plants by friction in the greenhouse. Purified virus was obtained by PEG precipitation and differential centrifugation. The virus contents were 21.42mg / 100g fresh weight and 24.50mg / 100g fresh weight respectively. The OD value was measured under UV light, which was the lowest at 248 nm (TMV) and 246 nm (PVX) respectively, the highest at 260 nm. Extinction coefficients OD260 / 280 were 1.20 and 1.22, respectively. The antigen was purified and returned to the test, which indicated that the plant developed obvious symptoms. The rabbit was immunized with Freund adjuvant in three times. The titer of antiserum obtained was 1: 2048. The globulin was extracted with (NH_4) _2SO_4 and labeled with HRP , Prepared antiserum has been applied to the production.