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TNF-α和IL-12是两种细胞因子,主要由巨噬细胞产生,在抵抗细胞内细菌感染中起重要作用。本研究用TNF-α和IL-12抗体的中和作用探索了内源性TNF-α和IL-12在抗细胞内感染中的作用。为了解TNF-α和IL-12对细菌数目减少的作用及对布氏杆菌感染的炎症应答,将实验分为3组。对照组为布氏杆菌感染鼠,试验组为TNF-α降低鼠和IL-12降低鼠。注射流产布氏杆菌19前4h,在鼠腹膜内分别给予试验组多克隆抗损TNF-α抗体、单克隆抗体抗鼠IL-12,使TINF-α或IL-12降低,加重流产布氏杆菌感染。鉴于在抗感染中IL-12降低的持续作用,6周后IL-12降低鼠的感
TNF-alpha and IL-12, two cytokines, are produced mainly by macrophages and play an important role in combating intracellular bacterial infections. This study explored the role of endogenous TNF-alpha and IL-12 in anti-intracellular infection using the neutralization of TNF-alpha and IL-12 antibodies. To understand the role of TNF-α and IL-12 in reducing the number of bacteria and the inflammatory response to brucellosis, the experiment was divided into three groups. The control group was brucellosis-infected mice, the experimental group was reduced TNF-α and IL-12 mice. The mice were injected intraperitoneally with polyclonal anti-TNF-α antibody and monoclonal antibody against murine IL-12 in the peritoneum of mice in the first 4 hours after injection of abortion brucine. The levels of TINF-α or IL-12 were decreased and Brucella abortus infection. IL-12 decreased the sense of the rat after 6 weeks, in view of the sustained effect of IL-12 reduction in anti-infectives