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目的了解吉林省珲春地区伯氏疏螺旋体与斑点热群立克次体的复合感染情况。方法运用PCR方法对吉林省珲春地区采集的蜱标本,进行伯氏疏螺旋体5S-23S rRNA间隔区基因与斑点热群立克次体外膜蛋白A(ompA)基因的检测。测序并用PHYLIP软件进行序列分析。结果全沟硬蜱中伯氏疏螺旋体感染率为36．0％,在全沟硬蜱中检测到了斑点热群立克次体的感染,其感染率为2．0％。二者的复合感染率为2．0％;森林革蜱中伯氏疏螺旋体感染率30．9％,斑点热群立克次体感染率29．1％,二者的复合感染率16．8％。伯氏疏螺旋体的序列分析显示吉林地区的伯氏疏螺旋体都属于B．garinii基因型,同源性较高。对斑点热阳性片段序列分析表明新测序列与斯洛伐克新发现的IRS3株和IRS4株核苷酸序列同源性为97％。结论吉林省珲春地区全沟硬蜱及森林革蜱中检测到伯氏疏螺旋体与斑点热群立克次体的感染,并检测到2种病原体的复合感染情况。
Objective To understand the combined infection of Borrelia burgdorferi and Rickettsia glabra in Hunchun area, Jilin province. Methods PCR was used to detect the 5S-23S rRNA spacer gene and speckled heat group ompA gene of ticks collected from Hunchun in Jilin Province. Sequencing and sequence analysis with PHYLIP software. Results The infection rate of Borrelia burgdorferi was 36.0% in hard ticks of Ictalurus punctatus, and the infection rate of Rickettsia speciosa was 2.0%. The combined infection rate was 2.0%. The infection rate of Borrelia burgdorferi in forest ticks was 30.9% and that of spotted fever group was 29.1%, the combined infection rate of both was 16.8 %. Sequence analysis of Borrelia burgdorferi showed that Borrelia burgdorferi in Jilin region belonged to B. garinii genotype, high homology. Sequence analysis of the hot spots showed that the nucleotide sequence homology between the new sequence and the newly discovered IRS3 and IRS4 strains in Slovakia was 97%. Conclusion The infection of Borrelia burgdorferi and Spotted fever group Rickettsia serrata in tick-hard Ixodidae and forest ticks in Hunchun, Jilin Province, was detected and the combined infection of two pathogens was detected.