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目的:优选神香草总黄酮的大孔树脂纯化工艺。方法:以总黄酮吸附率和洗脱率为指标,通过静态吸附-洗脱试验比较11种大孔树脂对神香草总黄酮的吸附和洗脱性能,筛选最佳大孔树脂型号。采用单因素试验考察上样液质量浓度、上样流速、树脂径高比、洗脱剂浓度及用量、洗脱流速等因素对大孔树脂纯化工艺的影响。结果:选用AB-8型大孔树脂,其最佳纯化工艺参数为上样液质量浓度2.56 g·L-1,上样流速4 BV·h-1,树脂径高比1∶5,加水5 BV以流速5 BV·h-1除杂,洗脱液弃去,加70%乙醇9 BV以流速4 BV·h-1洗脱,收集洗脱液。神香草总黄酮纯度达65.6%。结论:采用AB-8型大孔树脂纯化神香草总黄酮的工艺稳定可行,为该药用部位的开发提供参考。
Objective: To optimize the macroporous resin purification process of total flavonoids of Herba Vanilla. Methods: According to the adsorption and elution rates of total flavonoids, the adsorption and elution properties of 11 macroporous resins on total flavonoids of Herba Vannamei were compared by static adsorption-elution test, and the best macroporous resin model was screened. Single factor test was used to investigate the effects of factors such as mass concentration of sample solution, sample flow rate, resin diameter to height ratio, eluent concentration and dosage, elution flow rate on the purification of macroporous resin. Results: The optimum purification process parameters of AB-8 macroporous resin were as follows: the mass concentration of sample solution was 2.56 g · L-1, the flow rate of sample was 4 BV · h-1, the diameter-diameter ratio of resin was 1: 5, BV was purged with a flow rate of 5 BV · h-1, the eluate was discarded, and the eluate was collected by adding 9 BV of 70% ethanol at a flow rate of 4 BV · h-1. Shen vanilla flavonoids purity of 65.6%. Conclusion: The purification of total flavonoids from Herba Vanilla by AB-8 macroporous resin is stable and feasible, which can provide a reference for the development of this medicinal site.