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研究ＴＮＦ介导的ＢＣＭＥＣ释放ＰＤＧＦ及药物对ＢＣＭＳＭＣ增殖的保护作用．方法：体外培养ＢＣＭＥＣ和ＢＣＭＳＭＣ，用结晶紫染色法观察ＴＮＦ引起的ＢＣＭＥＣ释放ＰＤＧＦ继而促进ＢＣＭＳＭＣ增殖作用．结果：ＴＮＦ不能促进无血清培养的ＢＣＭＳＭＣ增殖，但ＴＮＦ（５－２０μｇ·Ｌ－１）剂量依赖地促进ＢＣＭＥＣ释放ＰＤＧＦ．ＴＮＦ（２０μｇ·Ｌ－１）促进ＢＣＭＳＭＣ增殖的百分率为３４±４％．药物Ｉｍｐｅｒａｔｏｒｉｎ，ｉｓｏｉｍｐｅｒａｔｏｒｉｎ和ＰＭＤＰ不影响ＴＮＦ引起ＢＣＭＥＣ释放ＰＤＧＦ，但能剂量依赖地（１－１００μｍｏｌ·Ｌ－１）抑制ＰＤＧＦ促ＢＣＭＳＭＣ增殖的作用．结论：ＴＮＦ促进ＢＣＭＥＣ释放ＰＤＧＦ继而引起ＢＣＭＳＭＣ的增殖．药物能抑制ＰＤＧＦ引起的ＢＣＭＳＭＣ增殖．
To study the protective effect of TNF-mediated release of PDGF by BCMEC and drug on the proliferation of BCMSMC. Methods: BCMEC and BCMSMC were cultured in vitro. Crystal violet staining was used to observe the release of PDGF by BCMEC induced by TNF and then the proliferation of BCMSMC. Results: TNF could not promote the proliferation of BCMSMC in serum-free medium, but TNF (5-20μg · L-1) promoted the release of PDGF in BCMEC in a dose-dependent manner. The percentage of proliferation of BCMSMC promoted by TNF (20μg · L-1) was 34 ± 4%. Drugs Imperatorin, isoimperatorin and PMDP did not affect TNF release of PDGF by BCMEC, but dose-dependently (1-100μmol·L-1) inhibited the proliferation of PDGF-stimulated BCMSMC. CONCLUSION: TNF promotes the release of PDGF by BCMEC and subsequently induces the proliferation of BCMSMC. Drugs can inhibit PDGF-induced BCMSMC proliferation.