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为研究支气管哮喘患者肺泡巨噬细胞（Ａｍ）所释放的内皮素１（ＥＴ１）在哮喘发病中的作用，对发作期哮喘患者２０例，对照组８例，经纤支镜行支气管肺泡灌洗术获得Ａｍ，调整Ａｍ至１×１０６／ｍｌ后分３孔体外培养（１ｍｌ／孔）；生理盐水对照孔、氟美松孔（１μｇ／ｍｌ）、氨茶碱孔（１５μｇ／ｍｌ），培养６ｈ后取上清液，采用放免法测定ＥＴ１。显示哮喘组血浆ＥＴ１明显高于对照组，并于第１秒用力肺活量（ＦＥＶ１．０）呈显著负相关。哮喘组Ａｍ体外释放ＥＴ１与对照组无显著差异，与ＦＥＶ１．０无相关性。氟美松孔Ａｍ释放ＥＴ１明显低于生理盐水对照孔；氨茶碱孔与生理盐水孔或氟美松孔无差异。提示哮喘Ａｍ所释放ＥＴ１在哮喘发病中意义不大
In order to study the role of endothelin-1 (ET-1) released by alveolar macrophages (Am) in the pathogenesis of asthma in patients with bronchial asthma, 20 patients with asthma in the attack phase, 8 patients in the control group, bronchus Am was obtained by alveolar lavage, and cultured in vitro (1 ml / well) in 3 wells after adjustment of Am to 1 × 10 6 / ml; normal saline control wells (1 μg / ml), aminophylline wells ), 6h after the supernatant was taken, using radioimmunoassay ET 1. ET 1 in asthma group was significantly higher than the control group, and forced vital capacity in the first second (FEV1.0) showed a significant negative correlation. Asthma group Am released in vitro ET 1 and the control group no significant difference, and FEV1.0 no correlation. Flumethasone hole Am release ET 1 was significantly lower than saline control hole; aminophylline hole and saline hole or the United States no difference between flumethasone. Prompted that the release of asthma ET 1 in the pathogenesis of asthma is of little significance