用异硫氰酸荧光素右旋糖酐-150作示踪剂,观察内毒素性休克时兔和大鼠微血管通透性变化。结果发现,内毒素性休克兔微血管通透性随休克进展进行性升高,血浆纤维连接蛋白(Fn)含量随之下降,两者呈显著相关(P<0.05)。在内毒素性休克大鼠中补充纯化大鼠Fn后,血浆Fn恢复,在静脉补充后2h内,微血管通透性升高受明显抑制,但2h后作用消失。与对照组(补充缺乏大鼠Fn血浆的内毒素性休克大鼠)相比,治疗组(补充纯化大鼠Fn)内毒素性休克大鼠的存活时间并无明显延长。 Fluorescein isothiocyanate-150 dextran was used as a tracer to observe the changes of capillary permeability in rabbits and rats during endotoxic shock. The results showed that the microvascular permeability of endotoxic shock rabbits increased progressively with the progress of shock, and the content of plasma fibronectin (Fn) decreased significantly (P <0.05). After Fn supplementation in rats with endotoxic shock, plasma Fn was restored. Increased capillary permeability was significantly inhibited within 2 h after intravenous infusion, but disappeared after 2 h. There was no significant prolongation of survival time in the treatment group (Fn supplemented with purified Fn) compared with the control group (endotoxic shock rats replenished with rat Fn plasma).