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目的探讨健脾化瘀方在体内抑制人胃癌MGC-803细胞侵袭转移的作用机制。方法建立裸鼠原位移植瘤模型,测算抑瘤率,并采用Real-time q PCR、Western Blot分别检测健脾化瘀方对侵袭转移相关基因和蛋白的影响。结果健脾化瘀方能抑制人胃癌MGC-803荷瘤裸鼠原位移植瘤生长;并能诱导原位移植瘤瘤体及肝转移灶MMP-2、MMP-9、MMP-14、VEGF基因表达下调,RECK基因表达上调;p-STAT3、MMP-2、MMP-9、MMP-14、VEGF蛋白表达量下降,RECK蛋白表达量上升,且均呈现量效关系。结论健脾化瘀方在体内环境下具有抑制人胃癌MGC-803侵袭转移的作用。
Objective To investigate the mechanism of Jianpi Huayu Recipe in inhibiting invasion and metastasis of human gastric cancer cell line MGC-803 in vivo. Methods The orthotopic transplantation tumor model was established in nude mice and the tumor inhibition rate was calculated. Real-time q PCR and Western Blot were used to detect the effect of Jianpi Huayu Recipe on the invasion and metastasis-related genes and proteins. Results Jianpi Huayu Recipe could inhibit the growth of orthotopically implanted human gastric cancer MGC-803 tumor-bearing nude mice and induce the expression of MMP-2, MMP-9, MMP-14 and VEGF in orthotopic transplantation tumor and liver metastases The expression of RECK was up-regulated; the expression of p-STAT3, MMP-2, MMP-9, MMP-14 and VEGF were decreased and the expression of RECK was up-regulated. Conclusion Jianpi Huayu Recipe can inhibit the invasion and metastasis of human gastric cancer MGC-803 in vivo.