本研究在已发现神经与肥大细胞之间有突触样联接关系的的基础上,检查了与肥大细胞形成联接的神经纤维终末内是否含有P物质和血管活性肠肽(VIP)。证明:光镜下P物质免疫反应阳性神经纤维与肥大细胞直接接触;将光镜看到的目的物用原位包埋法转到电镜下,可见P物质免疫反应轴索与肥大细胞紧密并靠,两者之间距离不超过20nm。含P物质免疫反应囊泡的轴突末梢在近肥大细胞处形成膨大,有的膨大部直接与肥大细胞接触,形成突触样联接。证实了前人关于与肥大细胞形成联接的轴突末梢内含有P物质的推断。此外,在光镜下证明的P物质和VIP免疫反应神经纤维在皮肤的分布与前人的报道一致,本实验并观察了它们在电镜下的结构特点。 This study examined the synaptic-like association between neuronal and mast cells and examined whether substance P and vasoactive intestinal peptide (VIP) are contained within the terminal nerve fibers that form mast cells. Proof: Under the light microscope, substance P-positive immunoreactive nerve fibers are in direct contact with mast cells; the target material seen by light microscopy is transferred to the electron microscope by in situ embedding method. It can be seen that substance P , The distance between the two does not exceed 20nm. The axons of the vesicle with substance P immunosuppression formed enlargement near the mast cells, and some enlargement directly contacted the mast cells to form synaptic-like connections. Confirming the predecessors’ inference of the substance P in axon terminals that form mast cells. In addition, the distribution of substance P and VIP immunoreactive nerve fibers in the skin demonstrated by light microscopy was consistent with previous reports, and their structural features under electron microscopy were observed.