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将苹果柱型基因的一个AFLP标记成功地转换成了简单实用的SCAR标记。首先对AFLP标记片段进行序列测定 ,然后根据序列特点设计了两对特异引物CoA1/CoA2 和CoA1/CoA3 ,每条引物长 2 0bp。PCR结果表明CoA1/CoA2 可以扩增出 2 16bp和 148bp两条带 ,其中 2 16bp的为柱型性状的特征带 ;CoA1/CoA3 可以扩增出 2 73bp和 2 0 5bp的两条带 ,其中 2 73bp的为柱型性状的特征带。两对引物在杂交后代中扩增出的特征带与柱型性状的分离重组率都很低 (CoA1/CoA2 为 6 .3%± 2 .5 % ;CoA1/CoA3 为 7.3%±2 .6 % ) ,所以它们都可以作为该SCAR标记的特异引物所用。
An AFLP marker of the apple column gene was successfully transformed into a simple and practical SCAR marker. First AFLP marker sequence was sequenced, and then according to the characteristics of the sequence designed two pairs of specific primers CoA1 / CoA2 and CoA1 / CoA3, each primer 20bp. The results of PCR showed that CoA1 / CoA2 could amplify two bands of 2 16 bp and 148 bp, of which 2 16 bp were characteristic bands of columnar traits. CoA1 / CoA3 could amplify two bands of 2 73 bp and 20 bp, of which 2 73bp is a characteristic band of columnar traits. The separation and recombination rates of characteristic bands and column traits amplified by the two pairs of primers were very low (6.3% ± 2.5% for CoA1 / CoA2 and 7.3% ± 2.6% for CoA1 / CoA3) ), So they can all be used as specific primers for this SCAR marker.