论文部分内容阅读
目的:探讨缺氧及在缺氧条件下运动对大鼠心肌代谢酶活性的影响。方法:24只Wistar大鼠随机分为4组:平原对照组、单纯缺氧组、平原运动组和缺氧复合运动组。缺氧复合运动组大鼠持续暴露于模拟海拔5000m低压舱内5周,每天将低压舱模拟海拔高度调至4000m进行游泳运动1小时(6天/周),运动结束后调至模拟海拔5000m;单纯缺氧组大鼠在低压舱内相同模拟海拔高度饲养,但不进行游泳运动;平原运动组和平原对照组在平原环境下饲养,其中平原运动组每天游泳运动1小时(6天/周)。实验5周后末次运动结束后24小时处死大鼠,分离左、右心室,称重并计算左、右心室重量指数。组织匀浆测定左、右心室己糖激酶(HK)、琥珀酸脱氢酶(SDH)、柠檬酸合成酶(CS)和乳酸脱氢酶(LDH)活性。结果:与平原对照组比较,单纯缺氧组和缺氧复合运动组大鼠右心室重量指数、Hermann-Willson指数显著增加,缺氧复合运动组大鼠左心室指数显著增高。单纯缺氧组大鼠左心室肌组织氧化酶SDH、CS活性降低,而缺氧复合运动组与对照组比无显著差异,缺氧复合运动组CS活性与缺氧组比较显著增加。与平原对照组比较,单纯缺氧组大鼠右心室HK、CS、LDH活性显著降低,缺氧复合运动组LDH活性较单纯缺氧组无显著差异,CS、SDH活性则显著增加。结论:长期缺氧使心肌组织氧化酶和糖酵解酶活性不同程度降低,缺氧复合运动后酶活性恢复或增加,表明在缺氧条件下适度运动,可调节有氧氧化和无氧酵解酶活性,增强机体对缺氧环境的代谢适应性。
Objective: To investigate the effects of hypoxia and exercise on hypoxia in myocardial metabolic enzymes. Methods: Twenty-four Wistar rats were randomly divided into 4 groups: plain control group, hypoxia group, plain exercise group and hypoxic combined exercise group. Rats in hypoxic combined exercise group were continuously exposed to simulated low altitude cabin of 5000m for 5 weeks. The simulated altitude of low pressure cabin was adjusted to 4000m for 1 hour (6 days / week), and the altitude was adjusted to 5000m after the end of exercise. The rats in the hypoxia group were fed at the same simulated altitude in the low-pressure cabin, but no swimming was carried out. The plain and plain control groups were kept in the plain environment, in which the plain exercise group swam for 1 hour (6 days / week) . Rats were sacrificed 24 hours after the last exercise after 5 weeks of experiment. Left and right ventricles were isolated, weighed and left and right ventricular mass index calculated. Left and right ventricular hexokinase (HK), succinate dehydrogenase (SDH), citrate synthase (CS) and lactate dehydrogenase (LDH) activity were measured by tissue homogenate. Results: Compared with plain control group, right ventricular weight index and Hermann-Willson index increased significantly in hypoxia-combined exercise group and hypoxic-combined exercise group, while left ventricular index increased significantly in hypoxic combined exercise group. The activities of SDH and CS in left ventricular myocytes of rats in hypoxia alone group were decreased, while those in hypoxia group were not significantly different from those in control group. The CS activity in hypoxia group was significantly increased compared with hypoxia group. Compared with the plain control group, the activities of HK, CS and LDH in the right ventricle of rats in the hypoxia group were significantly decreased, and the activity of LDH in the hypoxia combined exercise group was not significantly different from that of the hypoxia group. The activities of CS and SDH were significantly increased. CONCLUSION: Long-term hypoxia reduces the activity of oxidase and glycolytic enzyme in myocardial tissue to different extents, and the activity of the enzyme recovers or increases after hypoxia-combined exercise, indicating moderate exercise in hypoxia and regulating aerobic and anaerobic glycolysis Enzyme activity, enhance the body’s metabolic adaptation to hypoxic environment.