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目的研究碱性成纤维细胞生长因子(basic fibroblast growth factor,bFGF)及其受体(fibroblast growth factor receptor-1,FGFR-1)在正常及心肌梗死后心脏组织中的分布和变化情况,探讨bFGF在心肌梗死中对心脏的保护作用。方法普通级正常成年西双版纳微型猪11只,实验组(n=6)通过结扎成年微型猪的左冠状动脉前降支建立心肌梗死模型,对照组(n=5)模拟开胸手术过程,但不结扎冠状动脉,4周后取出心脏,采用免疫组化法和图像分析方法检测2组实验动物心脏组织中bFGF和FGFR-1的水平。结果①正常对照组的心房心肌细胞内bFGF和FGFR-1的蛋白表达水平高于心室(P<0.05)。②心肌梗死后,实验组左右室心肌组织的bFGF水平明显高于对照组(P<0.05),而以受累左室的增加最为明显。同时,受累左心室中的FGFR-1较对照组也升高(P<0.01),且以梗死边缘区最为显著(P<0.01)。③梗死区周围组织中的血管内皮细胞、平滑肌细胞内bFGF和FGFR-1表达较对照组增多(P<0.05)。结论心肌梗死后受累部位心肌bFGF明显增多,同时FGFR-1显著上调,推测bFGF在心肌梗死后的心脏血管新生、侧支循环建立、组织修复、心脏重构及改善心肌梗死预后方面可能起重要作用。
Objective To study the distribution and changes of basic fibroblast growth factor (bFGF) and its receptor (fibroblast growth factor receptor-1, FGFR-1) in cardiac tissue after normal and myocardial infarction Cardiac protection in myocardial infarction. Methods Eleven normal Xishuangbanna miniature pigs were enrolled in this study. The experimental group (n = 6) was established by ligating the left anterior descending coronary artery of adult miniature pigs. The control group (n = 5) simulated the thoracotomy but not Coronary artery was ligated and the heart was removed 4 weeks later. The levels of bFGF and FGFR-1 in heart tissue of two groups were detected by immunohistochemistry and image analysis. Results ① The protein expression of bFGF and FGFR-1 in atrial myocardium of normal control group was higher than that of ventricular (P <0.05). ② After myocardial infarction, the level of bFGF in the left and right ventricular myocardium in the experimental group was significantly higher than that in the control group (P <0.05), while the increase in the affected left ventricle was the most obvious. At the same time, FGFR-1 in the affected left ventricle also increased compared with the control group (P <0.01), and the most significant was the marginal zone of infarction (P <0.01). ③ The expression of bFGF and FGFR-1 in vascular endothelial cells and smooth muscle cells around the infarct area increased compared with the control group (P <0.05). Conclusions The myocardial bFGF increased significantly in myocardial infarction-affected area and FGFR-1 was up-regulated at the same time. It is speculated that bFGF may play an important role in angiogenesis, collateral circulation establishment, tissue repair, cardiac remodeling and myocardial infarction prognosis after myocardial infarction .