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目的:建立瘀血阻滞证糖尿病足大鼠动物模型。方法:给SD雄性大鼠腹腔注射链脲佐菌素后,降低环境温度,造成糖尿病足动物模型;同时监测各组大鼠的体重、饮水量、空腹血糖和血液流变学指标,观察各组大鼠舌下、耳廓脉络变化,进行各组大鼠肢端坏疽症状评分。结果:糖尿病足造模成功率75%。糖尿病足组与正常对照不降温组、降温组相比,体重明显减轻,饮水量明显增加,空腹血糖、肢端坏疽症状评分、血液流变学全血黏度低切、中切、高切、红细胞聚集指数均明显升高,舌质紫黯,舌下脉络增长增粗,耳廓苍白或紫黯。结论:本方法可成功建立瘀血阻滞证糖尿病足大鼠模型,为探讨糖尿病足的发病机理和筛选药物奠定基础。
Objective: To establish an animal model of blood stasis syndrome in rats with diabetic foot. Methods: Male SD rats were injected intraperitoneally with streptozotocin (STZ) to reduce the ambient temperature and cause diabetic foot animal model. At the same time, the body weight, water intake, fasting blood glucose and hemorheology of each group were observed. Rat sublingual, auricle vein changes, the group acral ganglion symptom score. Results: Diabetic foot model success rate of 75%. Diabetic foot group and normal control group did not cool, compared with the cooling group, body weight was significantly reduced, significantly increased drinking water, fasting blood glucose, acromegaly symptom score, hemorheology low blood viscosity whole cut, cut, high cut, red blood cells Aggregation index were significantly increased, dark purple tongue, thickened sublingual vein, auricle pale or dark purple. Conclusion: This method can successfully establish a rat model of stasis syndrome with blood stasis syndrome, and lay a foundation for exploring the pathogenesis of diabetic foot and screening drugs.