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目的··:调查重庆地区部分入住我院的静脉注射毒品者丙型肝炎病毒(HCV)不同基因型感染状况。方法·· :用酶标记免疫(ELISA)法检测HCV感染情况 ,用逆转录 -聚合酶链反应(RT -PCR)与限制性长度多态性(RFLP)分型检测HCVRNA ,并观察血清ALT、AST水平。结果··:入住我院的静脉注射毒品者中抗 -HCVIgG阳性率为40.5 %;HCVRNA阳性率为34.5 %,其中HCV1b型、2a型及1b/2a混合型感染率分别为33.7 %、46.9 %及19.4 %。在ALT/AST正常者和异常者之间 ,HCV各类型感染率无显著性差异。结论··:重庆地区部分静脉注射毒品者HCV感染以2a型为主 ,其次为1b型 ,1b/2a混合型亦不少见。HCV基因型与血清ALT和AST水平变化无明显相关性。
Objective · To investigate the infection status of different genotypes of hepatitis C virus (HCV) among intravenous drug users in our hospital in Chongqing. Methods · · · · · · ·: HCV infection was detected by enzyme-linked immunosorbent assay (ELISA) AST level. RESULTS: The positive rate of anti -HCVIgG was 40.5% and the positive rate of HCVRNA was 34.5% in the intravenous drug users admitted to our hospital. The mixed infection rates of HCV1b, 2a and 1b / 2a were 33.7% and 46.9% And 19.4%. There was no significant difference in HCV infection rates between normal and abnormal ALT / AST. Conclusions · · ·: HCV infection in some intravenous drug users in Chongqing area is mainly type 2a, followed by type 1b, 1b / 2a mixed type is not uncommon. There was no significant correlation between HCV genotype and serum ALT and AST levels.