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目的：建立间期荧光原位杂交技术，检测鼻咽癌细胞系和鼻咽癌石蜡切片中９ 号和３ 号染色体的数目改变情况。方法：中期染色体片及细胞系滴片制备，间期及石蜡切片荧光原位杂交。结果：９ 号染色体在鼻咽癌细胞系中为六倍体， 在石蜡包埋鼻咽癌组织中也呈多倍体改变。３ 号染色体在鼻咽癌细胞系中呈三倍体改变，在石蜡包埋鼻咽癌组织中则无非整倍性改变。结论：间期荧光原位杂交为一很有应用前景的技术，可以用于存档石蜡块的研究，考察染色体数目和／ 或特异基因改变情况，有一定诊断学意义，值得推广应用。
Objective: To establish a technique of interphase fluorescence in situ hybridization to detect the number changes of chromosome 9 and 3 in nasopharyngeal carcinoma cell lines and nasopharyngeal paraffin sections. Methods: Preparation of metaphase chromosomes and cell lines slides, interphase and paraffin sections by fluorescence in situ hybridization. Results: Chromosome 9 was hexaploid in nasopharyngeal carcinoma cell line and polyploidy in paraffin-embedded nasopharyngeal carcinoma. Chromosome 3 showed a triploid change in nasopharyngeal carcinoma cell lines and no change in aneuploidy in paraffin-embedded NPC tissues. Conclusions: Interphase fluorescence in situ hybridization is a promising technique that can be used to archive paraffin blocks, investigate the number of chromosomes and / or specific gene changes, and have some diagnostic significance and are worth popularizing and applying.