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优化测定三亚鲍鱼氨基酸试验条件。运用酸水解法处理样品,2,4-二硝基氟苯柱前衍生化,辅以高效液相色谱(HPLC)测定氨基酸含量;色谱条件为:色谱柱,Inertsil ODS-3 C_(18)柱(4.6 mm×150 mm,5μm);流动相A,含1%N,N-二甲基酰胺的0.05 mol/L醋酸钠-醋酸缓冲液(pH 6.4);流动相B,乙腈-水(1︰1,V/V),流动相A︰流动相B为70︰30(V/V);检测波长360 nm;流速1 mL/min;柱温33℃。结果发现,样品氨基酸总含量为808.73 mg/g。此方法样品处理充分彻底,操作简单,各氨基酸分离良好;与常规方法相比,HPLC法测定简便快速,精密度高,检出限低,回收率高,准确性好,结果令人满意。
Optimized determination of Sanya abalone amino acid test conditions. The samples were treated with acid hydrolysis, and the 2,4-dinitrofluorobenzene pre-derivatization was carried out. The content of amino acids was determined by high performance liquid chromatography (HPLC). The chromatographic conditions were as follows: column, Inertsil ODS-3 C 18 column (4.6 mm × 150 mm, 5 μm); mobile phase A, 0.05 mol / L sodium acetate-acetate buffer (pH 6.4) containing 1% N, : 1, V / V), mobile phase A: mobile phase B 70:30 (V / V); detection wavelength 360 nm; flow rate 1 mL / min; The results showed that the total amino acid content of the sample was 808.73 mg / g. Compared with the conventional method, the HPLC method has the advantages of simple and rapid determination, high precision, low detection limit, high recovery, good accuracy and satisfactory results.