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[目的]研究PML-RARα反义核酸(antisense oligodexynucleotide,ASODN)对硒化壳聚糖诱导的NB4细胞分化敏感性的影响。[方法]人工合成PML-RARαASODN经阳离子脂质体包裹后瞬时转染NB4细胞,采用RT-PCR检测转染后PML-RARα基因表达情况,通过镜下观察细胞形态改变,NBT法观察细胞功能变化及流式法检测细胞膜CD11抗原表达来观测细胞分化。[结果]经PML-RARαASODN处理的NB4细胞PML-RARα基因表达明显下调,PML-RARα基因的反义核酸和硒化壳聚糖均能提高细胞NBT还原率,升高CD11b抗原,二者合用,则NBT还原率和CD11b抗原表达进一步增强。[结论]阳离子脂质体转染PML-RARαASODN具有促进硒化壳聚糖诱导NB4细胞分化作用的敏感性。
[Objective] To investigate the effect of PML-RARα antisense oligodexynucleotide (ASODN) on chondroitinase-induced NB4 cell differentiation sensitivity. [Method] The PML-RARαASODN was transiently transfected into NB4 cells after being encapsulated by cationic liposomes. The expression of PML-RARα gene was detected by RT-PCR. The morphological changes of PML-RARα were observed by microscopy and the changes of cell function were observed by NBT method And flow cytometry to detect cell membrane CD11 antigen expression to observe cell differentiation. [Result] The expression of PML-RARα in NB4 cells treated with PML-RARαASODN was significantly down-regulated. Antisense nucleic acids and selenized chitosan of PML-RARα gene both increased the cell NBT reduction rate and raised the level of CD11b antigen. The NBT reduction rate and CD11b antigen expression are further enhanced. [Conclusion] The cationic liposome transfected PML-RARα ASODN has the sensitivity to promote the differentiation of NB4 cells induced by selenized chitosan.