论文部分内容阅读
A pot experiment combined with15 N isotope techniques was conducted to evaluate effects of the varying rates of urea.N fertilizer application on yields,quailty,and nitrogen use efficiency(NUE)of pakchoi cabbage(Brassica chinensis L.)and asparagus lettuce(Lactuca saiva L.).15 N-labbled urea(5.35 15 N atom%)was added to pots with 6.5kg soil of 0.14,0.18,0.21,0.25,and 0.29 g N/kg soil.and applied in two splits:60 percenl as basel dressing in the mixture and 40 percent as toodressing.The fresh yields of two vegetable species increased with the increasing input of urea-N,but there was a significant quadratic relationship between the dose of urea-N fertilizer application and the fresh yields.Whan the dosage of urea-N fertilizer reached a certain value,nitrate readily accumulated in the two kinds of plants due to the decrease in NR activity;furthermore,there was a linear nagative correlation between nitrate content and NR activity.With the increasing input of urea-N.ascorbic acid and soluble sugar initially increased,declined after a while,and crude fiber rapidly decreased too.Total absorbed N(TAN).N derived from fertilizer(Ndff),and N derived from soil(Ndfs)increased,and the ratio of Ndff and TAN also increased.but the ratio of Ndfs and TAN as well as NUE of urea-N fertilizer decreased with the increasing input of urea-N.These results suggested that the increasing application of labeled N fertilizer led lo the increase in unlabeled N(namely,Ndfs)presumably due to“added nitrogen interaction”(ANI),the decease in NUE of urea-N fertilizer may be due to excess fertilization beyond the levels of plant requirements and the ANI.and the decrease jn the two vege table yields with the increasing addition of urea-N possibly because the excess accumulation of nitrate reached a toxic level.