论文部分内容阅读
目的 :探索分叉双歧杆菌的完整肽聚糖 (WPG)的体内抑瘤途径。方法 :以激光共聚焦显微镜和免疫组化检测了大肠癌裸鼠移植瘤IκBα的含量以及NF κB的活化状况。结果 :大肠癌裸鼠移植瘤经WPG处理后 ,其IκBα的平均荧光强度明显高于肿瘤对照组 (P <0 0 1) ;而NF κB的活化状态则相反 ,WPG注射组大肠癌NF κB的阳性细胞密度显著低于肿瘤对照组 (P <0 0 1)。结论 :分叉双歧杆菌的WPG体内能明显抑制大肠癌IκBα的降解 ,最终阻抑NF κB的活化。
Objective: To explore the in vivo antitumor mechanism of intact peptidoglycan (WPG) of bifidobacterium bifidus. Methods: The expression of IκBα in colorectal carcinoma xenografts and the activation of NF κB were detected by confocal laser scanning microscopy and immunohistochemistry. Results: The average IκBα fluorescence intensity of the transplanted tumor of large intestine nude mice after WPG treatment was significantly higher than that of the tumor control group (P <0.01), while the activation state of NF κB was opposite. In WPG injection group, the expression of NF κB The positive cell density was significantly lower than that of the tumor control group (P <0.01). Conclusion: Bifidobacterium bifidum WPG in vivo can significantly inhibit the degradation of IκBα in colorectal cancer, eventually inhibiting the activation of NF κB.