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目的 探索全反式维甲酸 (RA)对神经干细胞和分化细胞P4 5 0RAI表达的影响。 方法 从大鼠胚胎脑中分离神经干细胞 ,用EGF促进其增殖 ,再用不同浓度的RA单独处理 ,在不同时间用RT PCR测定神经干细胞及分化细胞的P4 5 0RAI表达水平。 结果 1μmol LRA处理神经干细胞 ,P4 5 0RAImRNA水平最高。 1μmol L时2h就可检测到神经干细胞P4 5 0RAImRNA的生成 ,4～ 12h达到高峰 ,随后迅速下降 ,2 4h几乎检测不到P4 5 0RAImRNA。间断给予RA ,P4 5 0RAI的表达随之下降。在EGF诱导生成的分化细胞中无P4 5 0RAI表达 ,而在RA处理的分化细胞中有少量表达。 结论 神经干细胞表达P4 5 0RAI对RA有浓度依赖性并需要RA的持续存在 ,随着神经干细胞分化为神经细胞 ,其表达量下降
Objective To explore the effect of all-trans retinoic acid (RA) on the expression of P450RAI in neural stem cells and differentiated cells. Methods Neural stem cells (NSCs) were isolated from rat embryonic brain and stimulated with EGF. The cells were treated with different concentrations of RA. The expression of P450RAI in NSCs and differentiated cells was detected by RT PCR at different time points. Results 1 μmol LRA treatment of neural stem cells, P4 5 0RAI mRNA levels were the highest. After 1μmol L for 2h, the generation of P450RAI mRNA in neural stem cells could be detected and peaked at 4 ~ 12h, then decreased rapidly, and P450RAI mRNA was almost undetectable in 24 hours. Intermittent RA, P450RAI expression decreased. There was no P450RAI expression in the EGF-induced differentiated cells, but a small amount in the RA-treated differentiated cells. Conclusion The expression of P450RAI in neural stem cells has a concentration-dependent effect on RA and requires the persistence of RA. As the neural stem cells differentiate into neurons, the expression of P450RAI decreases