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目的:研究鬼针草总黄酮(TFB)对急性肝损伤小鼠的保护作用。方法:以D-半乳糖胺盐酸盐(D-GalN)ip建立小鼠急性肝损伤模型。小鼠随机分为5组:模型组、联苯双酯阳性药组(120 mg.kg-1)、TFB低、中、高剂量组(60,120,240 mg.kg-1),并设正常对照组。连续ig给药7 d后造模,24 h后测量各组小鼠胸腺、脾脏和肝脏指数。比色法检测血清中丙氨酸转氨酶(ALT)、门冬氨酸转氨酶(AST)以及碱性磷酸酶(AKP)活性,血清白蛋白(Alb)含量以及总抗氧化能力(T-AOC),Westernblot法检测肝脏中诱导型一氧化氮合酶(iNOS)的表达。HE染色观察肝组织病理学改变。结果:与模型组比较,TFB能明显提高D-GalN致急性肝损伤小鼠胸腺、脾脏、肝脏指数(P<0.01),同时降低血清中ALT,AST,AKP活性(P<0.01),并增加Alb,T-AOC含量(P<0.01),降低iNOS的表达(P<0.01),并减轻肝损伤程度。结论:鬼针草总黄酮对D-GalN所致急性肝损伤小鼠有一定保护作用,其机制可能与抗氧化作用和抑制细胞毒作用有关。
Objective: To study the protective effect of Bidens pilosa flavonoids (TFB) on acute hepatic injury in mice. Methods: A mouse model of acute liver injury was established by D-galactosamine hydrochloride (D-GalN) ip. The mice were randomly divided into 5 groups: model group, bifendate positive group (120 mg.kg-1), TFB low, medium and high dose group (60,120,240 mg.kg-1), and normal control group. After continuous ig administration for 7 days, the model of thymus, spleen and liver were measured 24 hours later. Serum alanine aminotransferase (ALT), aspartate aminotransferase (AST) and alkaline phosphatase (AKP) activity, serum albumin (Alb) and total antioxidant capacity (T-AOC) Western blotting was used to detect the expression of inducible nitric oxide synthase (iNOS) in the liver. Hepatic histopathological changes were observed by HE staining. Results: Compared with model group, TFB could significantly increase the index of thymus, spleen and liver (P <0.01) and ALT, AST and AKP in serum of D-GalN induced acute liver injury mice (P <0.01) Alb and T-AOC (P <0.01), and decreased the expression of iNOS (P <0.01), and alleviated the degree of liver injury. CONCLUSION: The total flavonoids of Bidens bipinnata can protect mice with acute hepatic injury induced by D-GalN. The mechanism may be related to its antioxidation and cytotoxicity.