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建立以超高效液相色谱-电喷雾串联质谱同时检测10种食品中的阿维菌素类药物(包括阿维菌素、伊维菌素、多拉菌素、依普菌素、莫西丁克和塞拉菌素6种大环内酯类药物)残留的确证方法。样品用乙腈提取,45℃旋蒸蒸干,再用乙腈-水(1:9,V/V)溶液复溶,PEP-C18混合固相萃取柱净化,氮气吹干后用乙腈定容,超高效液相色谱-串联质谱测定,电喷雾正离子(ESI+)、多反应模式下监测;结果表明,6种药物在5～250μg/kg范围内线性良好(R2>0.99),3个添加水平下,6种药物的回收率在60%～99%之间,相对标准偏差为0.2%～8.9%。6种药物的定量限(RSN≥10)均达5.0μg/kg,符合痕量分析的要求。
Avermectins (including abamectin, ivermectin, doramectin, eptifibatide, mecindatin And six antibiotics selamectin macrolide drugs) residual confirmation method. The sample was extracted with acetonitrile, evaporated to dryness at 45 ℃, reconstituted with acetonitrile-water (1: 9, V / V) solution, purified by PEP-C18 solid phase extraction column, High performance liquid chromatography-tandem mass spectrometry, electrospray positive ion (ESI +) and multi-reaction mode were performed. The results showed that there were good linearity (R2> 0.99) for the six drugs in the range of 5 ~ 250μg / The recoveries of six drugs ranged from 60% to 99% with relative standard deviations of 0.2% to 8.9%. The limits of quantitation (RSN≥10) of the six drugs reached 5.0 μg / kg, which met the requirements of trace analysis.