论文部分内容阅读
目的:研究山莨菪对培养神经细胞谷氨酶兴奋毒性的作用。方法:体外培养新生SD大鼠皮层神经细胞,在谷氨酸处理神经细胞前后分别加入10~(-6)、10~(-4)mol/L的山莨若碱,观察山茛菪碱对谷氨酸所致神经细胞损伤的作用及对乳酸脱氢酶(LDH)、一氧化氮(NO)、丙二醛(MDA)和超氧化歧化酶(SOD)含量与活性的影响。结果:山莨菪碱能显著改善神经细胞受损的形态结构,减少细胞死亡数,降低LDH和NO含量,抑制MDA生成,提高SOD活性,且效应与药物浓度成正比。结论:山莨菪碱对体外培养神经细胞谷氨酸兴奋毒性具有明显的保护作用,其机制可能与抗脂质过氧化有关。
Objective: To study the effect of anisodamine on the excitotoxicity of glutamate in cultured neurons. Methods: Cortical neurons of neonatal SD rats were cultured in vitro. 10 ~ (-6) and 10 ~ (-4) mol / L of scopolamine were added before and after glutamate treatment. The effect of anisodamine on Glutamate-induced neuronal injury and its effects on the content and activity of lactate dehydrogenase (LDH), nitric oxide (NO), malondialdehyde (MDA) and superoxide dismutase (SOD). Results: Anisodamine can significantly improve the morphological and structural damage of nerve cells, reduce the number of cell death, reduce the content of LDH and NO, inhibit the production of MDA and increase the activity of SOD, and the effect is proportional to the drug concentration. Conclusion: Anisodamine has a protective effect on glutamate excitotoxicity of cultured neurons in vitro. The mechanism may be related to anti-lipid peroxidation.