Optimization of Extraction Technology and Determination of Content of Total Phenols of Leaves in Aca

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[Objectives] To determine the optimum extraction technology for total phenols of leaves in Acanthopanax giraldii Harms.[Methods]The single factor test and orthogonal design were adopted to study the extraction technology for total phenols of leaves in Acanthopanax giraldii Harms. [Results]The optimum extraction conditions were determined as follows: extraction solvent( 70% methanol),solid-liquid ratio( 1∶ 35),and reflux extraction time( 60 min). Under these conditions,the yield of crude extract from total phenols of leaves in Acanthopanax giraldii Harms was 38. 67 mg/g.[Conclusions] The technology was simple,and of low cost and high extraction rate,suitable for the extraction of total phenols of leaves in Acanthopanax giraldii Harms. [Objectives] To determine the optimum extraction technology for total phenols of leaves in Acanthopanax giraldii Harms. [Methods] The single factor test and orthogonal design were adapted to study the extraction technology for total phenols of leaves in Acanthopanax giraldii Harms. [Results] The optimum extraction conditions were determined as follows: extraction solvent (70% methanol), solid-liquid ratio 1:35, and reflux extraction time 60 min. Under these conditions, the yield of crude extract from total phenols of leaves in Acanthopanax giraldii Harms was 38. 67 mg / g. [Conclusions] The technology was simple, and of low cost and high extraction rate, suitable for the extraction of total phenols of leaves in Acanthopanax giraldii Harms.
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